Journal: Cancers

Article Title: MiRNAs in Alcohol-Related Liver Diseases and Hepatocellular Carcinoma: A Step toward New Therapeutic Approaches?

doi: 10.3390/cancers15235557

Figure Lengend Snippet: Summary of deregulated miRNAs in ALD and cited in this review.

Article Snippet: MiR-10a , PI3K/AKT/mTOR , HCC patients, human HCC cell lines (Huh7, HepG2, and PLC) , Cell proliferation inhibition chemosensibility , Musashi 1 (MSI1) , [ ] .

Techniques: Expressing, Transformation Assay, Activation Assay, Migration

Journal: Cancers

Article Title: MiRNAs in Alcohol-Related Liver Diseases and Hepatocellular Carcinoma: A Step toward New Therapeutic Approaches?

doi: 10.3390/cancers15235557

Figure Lengend Snippet: ( A ) A transcriptomic dataset (GSE59492 from Gene Expression Omnibus Database) was used to analyze deregulated miRNAs between control and alcohol-related cirrhotic livers ( B ) A literature-based screening was used to classify them in oncomiRs or tumor suppressor miRNAs (miRsupressors). ( C ) Among deregulated miRNAs, some have unknown functions in HCC. ( D ) Overexpression of some of these microRNAs, such as miR-3622a, can be observed in HCC tumors (data retrieved from miRTV database in July 2023). ( E ) The same transcriptomic dataset (GSE59492) was used to compare deregulated miRNAs in alcohol-related cirrhosis with NASH related cirrhosis. Only two miRNAs (miR-4725 and miR-150) are commonly upregulated in both conditions, and one miRNA is commonly downregulated (miR-3162).

Article Snippet: MiR-10a , PI3K/AKT/mTOR , HCC patients, human HCC cell lines (Huh7, HepG2, and PLC) , Cell proliferation inhibition chemosensibility , Musashi 1 (MSI1) , [ ] .

Techniques: Gene Expression, Control, Over Expression

Journal: Cancers

Article Title: MiRNAs in Alcohol-Related Liver Diseases and Hepatocellular Carcinoma: A Step toward New Therapeutic Approaches?

doi: 10.3390/cancers15235557

Figure Lengend Snippet: Summary of deregulated miRNAs and their impacts on different pathways associated with HCC.

Article Snippet: MiR-10a , PI3K/AKT/mTOR , HCC patients, human HCC cell lines (Huh7, HepG2, and PLC) , Cell proliferation inhibition chemosensibility , Musashi 1 (MSI1) , [ ] .

Techniques: Inhibition, Cell Differentiation, Migration, Histone Deacetylase Assay, Blocking Assay

Journal: Cancers

Article Title: MiRNAs in Alcohol-Related Liver Diseases and Hepatocellular Carcinoma: A Step toward New Therapeutic Approaches?

doi: 10.3390/cancers15235557

Figure Lengend Snippet: ( A ) A transcriptomic dataset (GSE10694) was used to identify new miRNAs deregulated in hepatocellular carcinoma with alcohol abuse. The data are represented in a heatmap showing the log2 fold change of deregulated miRNAs. ( B ) Significantly deregulated microRNAs were subjected to literature-based screening to classify them in the most common HCC-related pathways. The data were retrieved in July 2023. miRNAs in red bubbles: upregulated; miRNA in blue bubbles: downregulated. Black arrows: activation of the pathway; Blue inhibitory arrows: pathway inhibition. Created with Biorender.com .

Article Snippet: MiR-10a , PI3K/AKT/mTOR , HCC patients, human HCC cell lines (Huh7, HepG2, and PLC) , Cell proliferation inhibition chemosensibility , Musashi 1 (MSI1) , [ ] .

Techniques: Activation Assay, Inhibition

Journal: The Journal of International Medical Research

Article Title: The combination of ulinastatin and 5-fluorouracil synergistically inhibits hepatocellular carcinoma growth

doi: 10.1177/0300060520909776

Figure Lengend Snippet: 5-FU-resistant HCC cells exhibited increased stemness compared with parental cells. (a and b) The IC 50 values of 5-FU in Huh7-FT and Huh7 cells. (c and d) Sphere-formation ability of Huh7-FT and Huh7 cells. (e) ALDH1 activity was determined in Huh7 and Huh7-FT cells. (f and g) The expression of stemness regulators were measured in Huh7 and Huh7-FT cells. *P<0.01. 5-FU, 5-fluorouracil; HCC, hepatocellular carcinoma; ALDH1, aldehyde dehydrogenase.

Article Snippet: The HCC cell line Huh7 was purchased from Sino Biological (Beijing, China) and cultured in DMEM (Thermo Fisher Scientific, Waltham, MA, USA) with 15% fetal bovine serum (FBS; Sciencell, Carlsbad, CA, USA) at 37°C in 5% CO 2 .

Techniques: Activity Assay, Expressing

Journal: The Journal of International Medical Research

Article Title: The combination of ulinastatin and 5-fluorouracil synergistically inhibits hepatocellular carcinoma growth

doi: 10.1177/0300060520909776

Figure Lengend Snippet: UTI attenuated the stemness of 5-FU-resistant HCC cells. (a and b) Sphere-formation ability was evaluated in Huh7-FT cells treated with different concentrations of UTI. (c) ALDH1 activity was measured in Huh7-FT cells treated with different concentrations of UTI. (d and e) The expression of stemness regulators was detected in Huh7-FT cells treated with different concentrations of UTI. (f and g) Cell viability was examined in the cells described in (a). *P<0.05, **P<0.01. UTI, ulinastatin; 5-FU, 5-fluorouracil; HCC, hepatocellular carcinoma; ALDH1, aldehyde dehydrogenase.

Article Snippet: The HCC cell line Huh7 was purchased from Sino Biological (Beijing, China) and cultured in DMEM (Thermo Fisher Scientific, Waltham, MA, USA) with 15% fetal bovine serum (FBS; Sciencell, Carlsbad, CA, USA) at 37°C in 5% CO 2 .

Techniques: Activity Assay, Expressing

Journal: The Journal of International Medical Research

Article Title: The combination of ulinastatin and 5-fluorouracil synergistically inhibits hepatocellular carcinoma growth

doi: 10.1177/0300060520909776

Figure Lengend Snippet: UTI attenuated the 5-FU resistance of 5-FU-resistant HCC cells. (a and b) Cell viability was determined in Huh7 and Huh7-FT cells treated with 5-FU with and without UTI. (c and d) Levels of the apoptotic executors cleaved caspase 3 and cleaved PARP were examined in the cells described in (a). **P<0.01. UTI, ulinastatin; 5-FU, 5-fluorouracil; HCC, hepatocellular carcinoma.

Article Snippet: The HCC cell line Huh7 was purchased from Sino Biological (Beijing, China) and cultured in DMEM (Thermo Fisher Scientific, Waltham, MA, USA) with 15% fetal bovine serum (FBS; Sciencell, Carlsbad, CA, USA) at 37°C in 5% CO 2 .

Techniques:

Journal: The Journal of International Medical Research

Article Title: The combination of ulinastatin and 5-fluorouracil synergistically inhibits hepatocellular carcinoma growth

doi: 10.1177/0300060520909776

Figure Lengend Snippet: UTI suppressed Wnt/β-catenin signaling in 5-FU-FT cells. (a and b) Wnt3a and β-catenin expression were detected in Huh7-FT cells treated with different concentrations of UTI. (c and d) The expression of downstream β-catenin effectors were examined in Huh7-FT cells treated with different concentrations of UTI. **P<0.01. UTI, ulinastatin; 5-FU, 5-fluorouracil.

Article Snippet: The HCC cell line Huh7 was purchased from Sino Biological (Beijing, China) and cultured in DMEM (Thermo Fisher Scientific, Waltham, MA, USA) with 15% fetal bovine serum (FBS; Sciencell, Carlsbad, CA, USA) at 37°C in 5% CO 2 .

Techniques: Expressing

Journal: The Journal of International Medical Research

Article Title: The combination of ulinastatin and 5-fluorouracil synergistically inhibits hepatocellular carcinoma growth

doi: 10.1177/0300060520909776

Figure Lengend Snippet: UTI attenuated the stemness and 5-FU resistance of 5-FU-resistant HCC cells by modulating Wnt/β-catenin signaling. (a and b) Sphere-formation ability was evaluated in Huh7-FT cells treated with UTI with and without SKL2001. (c) ALDH1 activity was measured in Huh7-FT cells treated with UTI with and without SKL2001. (d and e) The expression of stemness regulators were examined in Huh7-FT cells treated with UTI with and without SKL2001. (f and g) The viability of Huh7 and Huh7-FT cells treated with 5-FU and UTI with and without SKL2001. **P<0.01. UTI, ulinastatin; 5-FU, 5-fluorouracil; HCC, hepatocellular carcinoma; ALDH1, aldehyde dehydrogenase.

Article Snippet: The HCC cell line Huh7 was purchased from Sino Biological (Beijing, China) and cultured in DMEM (Thermo Fisher Scientific, Waltham, MA, USA) with 15% fetal bovine serum (FBS; Sciencell, Carlsbad, CA, USA) at 37°C in 5% CO 2 .

Techniques: Activity Assay, Expressing

Journal: Therapeutic Advances in Medical Oncology

Article Title: Split chimeric antigen receptor-modified T cells targeting glypican-3 suppress hepatocellular carcinoma growth with reduced cytokine release

doi: 10.1177/1758835920910347

Figure Lengend Snippet: Split anti-hGPC3 CAR-T cells have potent and specific cytotoxicity against hGPC3 + HCC cell lines. 293T, HepG2, and Huh7 cells were cocultured with Mock-T, conventional anti-hGPC3 CAR-T, and SpyCatcher CAR-T cells with or without 10 nM anti-hGPC3 scFv-SpyTag in 96-well plates at different E:T ratios for 24 h. (a) Pictures were taken under an inverted optical microscope at 200x magnification field of view. Scale bar, 100 μm. (b) Culture supernatants were collected, and the killing effect of each well was calculated using a LDH-based cytotoxicity assay kit according to the manufacturer’s instruction. (c) HepG2 and Huh7 cells were cultured alone or cocultured with SpyCatcher CAR-T cells at an E:T ratio of 9:1 followed by the addition of a serial diluted anti-hGPC3 scFv-SpyTag. After 24 h coculture, supernatants were collected and cytotoxicities determined by LDH-based assays. Experiments were repeated twice with three replicates each. Repeated-measures ANOVA was used to compare different groups. ANOVA, analysis of variance; CAR-T, chimeric antigen receptor T; E:T, effector:target; HCC, hepatocellular carcinoma; hGPC3, human glypican-3; LDH, lactose dehydrogenase; SD, standard deviation.

Article Snippet: Human HCC Huh7 cells were purchased from Sangon Biotech (#E680517, Shanghai, China).

Techniques: Microscopy, LDH Cytotoxicity Assay, Cell Culture, Standard Deviation